Alimova T. S., Sivolapov V. A., Karpechenko N. A., Shishkina O. K., Panteleev S. V., Kovalevich O. A. Molecular Genetic Methods Implementation for Phytopathogen Identification in Forest Stands and Nurseries of the Russian Federation
2 Russian Centre for Forest Protection, Centre for Forest Protection of Voronezh Oblast
3 Institute of Forest, The National Academy of Sciences of Belarus
E-mail: shok3011@yandex.ru, vladimir-sivolapov@yandex.ru, nikitakarpechenko@mail.ru, shok3011@yandex.ru, stasikdesu@mail.ru, o-kovalevich@mail.ru
Abstract
How to cite: Alimova T. S.1, Sivolapov V. A.2, Karpechenko N. A.2, Shishkina O. K.1, Panteleev S. V.3, Kovalevich O. A.3 Molecular genetic methods implementation for phytopathogen identification in forest stands and nurseries of the Russian Federation // Sibirskij Lesnoj Zurnal (Siberian Journal of Forest Science). 2014. N. 4: 35–41 (in Russian with English abstract).
© Alimova T. S., Sivolapov V. A., Karpechenko N. A., Shishkina O. K., Panteleev S. V., Kovalevich O. A., 2014
Molecular analysis methods of pathogens have ceased to be unusual and are recognized as tools for microbiologists working in medical and epidemiological diagnosis, as well as biotechnology. These methods allow you to quickly determine the presence of infection, the identity of the pathogen and the molecular characteristics causing antibiotic resistance, to predict the infectivity, to identify the source of infection, or to establish the right to use the strain information in studies. It is worth noting that in the forest phytopathology, these methods are not yet widely used. Assessing the diversity of microflora using universal markers of the "barcode of the life" as well as a strain-specific marker allows you to solve a wide range of tasks in forest phytopathology. As is the case with medical microbiology, use of molecular techniques will allow study of the causes of diseases of the forest, their propagation dynamics, assessment of the biological diversity of the pathogen resistance to the methods used to combat the disease and the best strategy to improve the phytosanitary situation and agrotechnics. The prospects for using molecular techniques explained speed of implementation, efficiency, scalability and adaptability for any task of forest science and production. The results of the application of molecular genetics methods for the analysis of plant pathogen presence in forest plantations and nurseries of the Russian Federation, including doughnut fungus and annosum root rot, are presented. The prospects and benefits of using DNA analysis for early diagnosis of plant diseases without isolation of the pathogen in pure culture, shortening time of analysis, and the possibility of mass screening are discussed.
