Shigapov Z. Kh., Putenikhina K. V., Shigapova A. I., Urazbakhtina K. A., Putenikhin V. P. Genetic Diversity of the Siberian Stone Pine under Introduction in the South Urals and Bashkir Cis-Urals

Abstract

How to cite: Shigapov Z. Kh., Putenikhina K. V., Shigapova A. I., Urazbakhtina K. A., Putenikhin V. P. Genetic diversity of the Siberian stone pine under introduction in the South Urals and Bashkir Cis-Urals // Sibirskij Lesnoj Zurnal (Siberian Journal of Forest Science). 2016. N. 5: 137–146 (in Russian with English abstract).

DOI: 10.15372/SJFS20160515

© Shigapov Z. Kh., Putenikhina K. V., Shigapova A. I., Urazbakhtina K. A., Putenikhin V. P., 2016

Allozyme polymorphism of Siberian stone pine (Pinus sibirica Du Tour) has been studied in 14 artificial stands in the South Urals and Bashkir Cis-Urals on the base of 7 gene-enzyme system analysis. The following values of genetic diversity are determined: mean number of alleles per locus (A) constitutes 1.69 ± 0.08; portion of polymorphic loci (P₉₅) – 50.0 %; the average expected heterozygosity (H_e) – 0.121 ± 0.015; the average observed heterozygosity (H_o) – 0.127 ± 0.017.The level of genetic variability in artificial stands of Siberian stone pine in the region is somewhat inferior to that in natural populations of the species. The highest genotype heterozygosity is determined in high-productive 110 year-old artificial stand in the South Urals (Beloretsky-2 site), and also in Ufimsky and Mishkinsky sites in Bashkir Cis-Urals. The lowest heterozygosity values are revealed in Birsky and Tuimazinsky sites characterized by the weakened vital state of individuals. In total we can speak about the maintenance of essential parts of the species’ genetic polymorphism under introduction, especially in some stands. Genetic similarity of the studied stands is shown: inter-sample component of the total genetic diversity (F_ST) constitutes 2.2 %, the average Nei’s genetic distance (D) – 0.0033 ± 0.00023, that is also typical of natural populations of Siberian stone pine in the species range. The obtained data about the genetic variability level of artificial stands in a complex with forestry characteristics give evidence of the successful species introduction in the region and the necessity of resumption of works on Siberian stone pine culture establishment in an industrial scale.