Shilkina E. A., Sheller M. A., Razdorozhnaya T. Y., Ibe A. A. DNA Diagnostic Results of Forest Nurseries Phytopathogenic Fungi of Krasnoyarsk Krai and the Republic of Khakassia

The DNA-diagnostic results of phytopathogens in 26 forest nurseries of Krasnoyarsk Krai and the Republic of Khakassia in the period from 2014 to 2016 were presented. The objects of research were the plants of conifer species Pinus sylvestris L., Pinus sibirica Du Tour and Picea obovata Ledeb. aged from 1 to 6 years. According to the data of molecular genetic analysis in the forest nurseries of the studied areas, the representatives of 13 genera of pathogenic and conditionally pathogenic fungi that cause diseases of conifers were revealed: Phoma Sacc., Didymella Sacc., Alternaria Nees, Cladosporium Link, Rhizoctonia DC., Lophodermium Chevall., Gremmenia Korf. (Phacidium Fr.), Sclerophoma Höhn. (teleomorph – Sydowia Bres.), Typhula (Pers.) Fr., Botrytis P. Micheli ex Pers., Gremmeniella M. Morelet (Scleroderris (Fr.) Bonord.), Septorioides Quaedvl., Verkley & Crous and Epicoccum Link. Seven genera of micromycetes are found in all three surveyed forest zones: taiga, forest-steppe and Southern-Siberian mountain. The most frequently occurred lesions were caused by the sac fungi. Some of the identified diseases practically had not been diagnosed before. In each of the nurseries, the pathogenic microflora was represented by 1-6 species of microscopic fungi, several pathogens were often presented simultaneously on the infected seedlings. In the process of working, the dominant pathogens, connection of pathogens with certain species and with the seedlings age composition have been identified. Such diseases as Phoma blight and Lophodermium needle cast predominated the frequency of occurrence, their relative abundance was 30 % and 28 % respectively of all identified fungal infections of plants. Also, given the climatic conditions of the region, attention should be paid to the phytopathogens – Gremmenia infestans (P. Karst.) Crous and Typhula sp.