Tikhonova I. V., Semerikov V. L., Semerikova S. A., Dymshakova O. S., Zatcsepina K. G. About Samples in the Research of Intraspecific Genetic Diversity of Scots Pine (Pinus sylvestris L.)

Abstract

How to cite: Tikhonova I. V.¹, Semerikov V. L.², Semerikova S. A.², Dymshakova O. S.², Zatcsepina K. G.¹ About samples in the research of intraspecific genetic diversity of Scots pine (Pinus sylvestris L.) // Sibirskij Lesnoj Zurnal (Siberian Journal of Forest Science). 2014. N. 4: 99–109 (in Russian with English abstract).

© Tikhonova I. V., Semerikov V. L., Semerikova S. A., Dymshakova O. S., Zatcsepina K. G., 2014

The influence of sample size on the results of the assessments of the genetic diversity of populations of Scots pine of 12 polymorphic loci allozymes and 4 chloroplast microsatellite loci (cpSSR) was investigated. According to the obtained results, the use of different genetic markers gives similar estimates of the proportion of species intrapopulation diversity. The contribution of individual variation within populations (Fst) was 98.5 % for the allozyme data and 97.9 % on cpSSR DNA markers. It was found that samples of more than 100 trees are characterized by a higher diversity of alleles. Shown was a logarithmic relationship between the number of identified chloroplast haplotypes and the volume and the number of samples in the studying area (correlation coefficient r = 0.983). An increase of up to 100 and more sample trees leads to reduction of genetic interpopulation distance Nei (1972, 1978) without significant change in the parameters characterizing the genetic structure of the species. Standard sample sizes (30-40 individuals) allow reliable estimates of the population genetic structure of the species, but not enough to study its intra-population diversity. The results suggest the need to preserve the gene pool of species over large areas and the desirability of increasing the sample geographic populations to take into account the greatest amount of genetic polymorphism of species.