Makarov S. S., Upadyshev M. T., Rodin S. A., Makarova T. A., Samoylenko Z. A., Kuznetsova I. B. Adaptation of Regenerated Plants of Rubus arcticus L. to ex vitro Conditions Using Hydroponics
1 Russian Timiryazev State Agrarian University
Timiryazevskaya Str., 49, Moscow, 127434 Russian Federation
2 Northern (Arctic) Federal University named after M. V. Lomonosov
Naberezhnaya Severnoy Dviny, 17, Arkhangelsk, 163002 Russian Federation
3 Federal Horticultural Center for Breeding, Agrotechnology and Nursery
Zagor’evskaya Str., 4, Moscow, 115598 Russian Federation
4 All-Russian Research Institute of Silviculture and Mechanization of Forestry
Institutskaya str., 15, Pushkino, Pushkinsky District, Moscow Oblast, 141202 Russian Federation
Prospekt Lenina, 1, Surgut, Khanty-Mansi Autonomous Okrug – Yugra, 628412 Russian Federation
6 Kostroma State Agrarian Academy
Uchebny Gorodok str., 34, Karavaevskaya s/a, Karavaevo, Kostromskoy District, Kostroma Oblast,
156530 Russian Federation
E-mail: makarov_serg44@mail.ru, virlabor@mail.ru, info@vniilm.ru, tatiana.makarowa2010@yandex.ru,
Abstract
UDC 634.7:631.589.2
How to cite: Makarov S. S.1, 2, Upadyshev M. T.3, Rodin S. A.4, Makarova T. A.5, Samoylenko Z. A.5, Kuznetsova I. B.6 Adaptation of regenerated plants of Rubus arcticus L. to ex vitro conditions using hydroponics // Sibirskij Lesnoj Zurnal (Sib. J. For. Sci.). 2023. N. 4. P. 75–82 (in Russian with English abstract and references).
DOI: 10.15372/SJFS20230408
© Makarov S. S., Upadyshev M. T., Rodin S. A., Makarova T. A., Samoylenko Z. A., Kuznetsova I. B., 2023
The results of studies on the adaptation of arctic bramble (Rubus arcticus L.) plants of Astra and Sofia cultivars obtained by the clonal micropropagation method to non-sterile ex vitro conditions using the hydroponic method of cultivation are discussed in the article. The arctic bramble is a highly valuable species of forest berry plants in food and medicinal relations, rarely found and having a low yield in natural conditions. Adaptation to non-sterile ex vitro conditions is one of the limiting stages in clonal micropropagation. The survival rate of regenerated plants of R. arcticus of the studied cultivars in the conditions of a hydroponic system is 98 %, 1 month after planting in open ground – 69 %. Regenerated plants of R. arcticus have stronger differences in morphometric parameters between clones within a cultivar than between cultivars. R. arcticus planted in conglomerates formed shoots in the amount of 5.0 pcs. with an average length of 2.5 cm on the 20th day of adaptation. On the 45th day R. arcticus plants after division into separate shoots had an average of 3.0 pcs. shoots with an average length of 8.3 cm. R. arcticus plants of the Sofia cultivar have higher morphometric parameters of the above-ground and root systems compared to the Astra cultivar on the 45th day of cultivation in a hydroponic system: by the number of roots – by 30.3 %, by the total length of the roots – by 23 %, by the number of leaves – by 20 %, by the number of shoots – by 14 %, by the length of the shoots – by 10 %. The indicators of the development of the root system of R. arcticus on a hydroponic installation depended on the varietal characteristics and the growing period. R. arcticus plants have roots 10–20 cm long and shoot growths of 20–30 cm after 2 months of adaptation, which indicates its suitability for planting in open ground conditions.
Article
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